RNASeq of ABC07: Penicillin replicate 3 - SRA

SRX2918662: RNASeq of ABC07: Penicillin replicate 3
1 ILLUMINA (Illumina HiSeq 2000) run: 7.3M spots, 307.6M bases, 192.5Mb downloads

Design: Cell pellets stored in Qiagen RNAProtect reagent, DNA extracted by phenol:chloroform, rRNA depleted by Epicentre Ribo-Zero rRNA removal kit, cDNA prepared by Invitrogen SuperScript II, sheared by Covaris S2, and sequenced 1 x 50 bp by Illumina HiSeq2000

Submitted by: Washington University in Saint Louis

Study: Penicillin consuming ABC strain genomes

PRJNA385617 • SRP106815 • All experiments • All runs

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The goal of this project is to identify the gene pathways and enzyme mechanisms used by penicillin-consuming bacteria during growth on penicillin as a sole carbon source. These bacteria represent an under studied component of soil antibiotic ecology and utilize unknown enzymes in their metabolisms.

Sample:

SAMN06915398 • SRS2177241 • All experiments • All runs

Organism: Pseudomonas sp. PE-S1G-1

Library:

Name: ABC07_PE_3

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: SINGLE

Runs: 1 run, 7.3M spots, 307.6M bases, 192.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR5683529	7,323,904	307.6M	192.5Mb	2018-05-05

ID:: 4171948

SRA

Sequence Read Archive

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